بررسی فنوتیپیک زیر گروههای لنفوسیتی خون محیطی بیماران مبتلا به درماتیت تماسی آلرژیک و مقایسه آن با سایر درماتیتها و افراد سالم

Contact dermatitis is seen in two forms of allergic contact dermatitis (ACD) with 20% and irritant contact dermatitis (lCD) with SO% Frequency. This disease is manifested in three forms of acute, sub-acute, and chronic, characterized by pain, itching, vesicle, swelling, papul, lichenification and oozing. ACD or contact hypersensitivity (CHS) in an eczematous dermatitis which is regarded as T-cell mediated delayed - type hypersensitivity (DTH) reaction, caused by exposure to some substances. Those substances act as haptenic allergens. The role ofT- Lymphocyte subsets has been clearly shown in the pathogenesis of this disease, However, results from different studies on blood samples are contraversial in this regard. This project aimed at a broader study on distribution of different lymphocyte subsets in peripheral blood of patients with CBS compared with other types of dermatitis and normal individuals. Patch - Test is generally required to identify the specific allergen(s). In this study, we used Europen srandard patch test kit (Trolab) with 23 allergens, tested 46 patients and 36 control healthy individuals. Forty - eight hours later, 5 ml peripheral blood sample was obtained from the patient and control groups. Serum total IgE of patient and control groups with ELISA were assessed. Peripheral blood samples were stained with mAbs (lMK-Plus Kit) Conjugated with FITC and PE, such as CD 14/45, (CD3+, CD4+, CDS+) T, (CD 16+ / CD 56) NK, (HLA - DR) T and (CD19) B, and analyzed on F ACS-Calibur (Becton Dickinson Company) Flowcytometry. The results of patch-Test revealed that the most frequent positive reactions were due to nickel - sulphate, cobalt choride, potasium dichromat and formaldehyde, The results of serum total IgE in ACD and ICD showed nonsignificant difference from control group. In patients with atopic dermatitis total IgE was S to 10 times greater than serum IgE in control group (P<O.OO 1). Mean percent, total T-cells (CD3+) and (CD4) T-cell subset showed nonsignificant change compared to control group (CDS) T-cell subsets showed some increase and total B-cells (CD19) some decrease which were nonsignificant.